Determination of the Content of Meropenem and Observation of the Stability of Meropenem in Mixing With Infusion Fluids / 中国药房

OBJECTIVE:To establish a HPLC method for determining the content of Meropenem and to observe the stability of Meropenem in mixing with 5%GS,GNS and NS.METHODS:The conditions of HPLC were:column,Nova-Pak C18;mobile phase,acetonitrile-0.05mol KH2PO4(4∶96);flow rate,1.0ml/min;detection wavelength,300nm.After Meropenem mixing with 5%GS,GNS and NS,the changes of external apperance were observed in room temperature,the content was detected with HPLC and the pH and insoluble particles were determined.RESULTS:The calibration curve was in good linearity in the range of 12.5～250?g/ml(r=0.9 999)and the recovery was 99.7% with RSD=1.1%.CONCLUSION:This detection method is effective,rapid and accurate.Meropenem is stable in infusion fluids for 4 hr.